BioNano genome mapping of individual chromosomes supports physical mapping and sequence assembly in complex plant genomes
نویسندگان
چکیده
منابع مشابه
BioNano genome mapping of individual chromosomes supports physical mapping and sequence assembly in complex plant genomes
The assembly of a reference genome sequence of bread wheat is challenging due to its specific features such as the genome size of 17 Gbp, polyploid nature and prevalence of repetitive sequences. BAC-by-BAC sequencing based on chromosomal physical maps, adopted by the International Wheat Genome Sequencing Consortium as the key strategy, reduces problems caused by the genome complexity and polypl...
متن کاملSequence-based physical mapping of complex genomes by whole genome profiling.
We present whole genome profiling (WGP), a novel next-generation sequencing-based physical mapping technology for construction of bacterial artificial chromosome (BAC) contigs of complex genomes, using Arabidopsis thaliana as an example. WGP leverages short read sequences derived from restriction fragments of two-dimensionally pooled BAC clones to generate sequence tags. These sequence tags are...
متن کاملPhysical mapping of human chromosomes by repetitive sequence fingerprinting.
We have developed an approach for identifying overlapping cosmid clones by exploiting the high density of repetitive sequences in complex genomes. Individual clones are fingerprinted, using a combination of restriction enzyme digestions followed by hybridization with selected classes of repetitive sequences. This "repeat fingerprinting" technique allows small regions of clone overlap (10-20%) t...
متن کاملPhysical Mapping of Bacterial Genomes
The use of physical maps for the description and comparison of bacterial genomes has dramatically increased in the last few years with the advent of powerful tools for the construction of physical maps of large DNA molecules. The availability of restriction enzymes that cut whole bacterial genomes only a few times together with the use of pulsed-field gel electrophoresis to resolve and size the...
متن کاملAssembly and analysis of complex plant genomes
Because the bulk of the maize (Zea mays L.) genome consists of repetitive sequences, sequencing efforts are being targeted to its “gene-rich” fraction. Traditional assembly programs are inadequate for this approach because they are optimized for a uniform sampling of the genome and inherently lack the ability to differentiate highly similar paralogs. Here, we report the development of bioinform...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Biotechnology Journal
سال: 2016
ISSN: 1467-7644
DOI: 10.1111/pbi.12513